Seed Priming Evidence Atlas

WG1-01 / RESOURCE SERIES

Seed Priming
Evidence Atlas

A visual introduction to treatments, plant systems, stresses and omics evidence

PrimSeedPower CA24156 · WG1 Deliverable D1 (Month 12)
Public-facing draft · database QA and WG1 approval pending

At a glance

The WG1 evidence map brings together published original research on molecular responses to seed priming. It shows where research has accumulated and where evidence remains sparse.

193Unique articles
53Plant species
5Omics domains

Study frequencies describe research coverage. They cannot establish causality, treatment efficacy or validated universal biomarkers.

How WG1 built the map

WG1 organized searches across four subgroups, followed by staged screening, structured extraction and G5 harmonization. The March 2026 search covered original articles from 2010 onward indexed in Scopus and/or Web of Science.

D1 screening snapshot
1,069 search records → 475 deduplicated → 260 full-text candidates → 204 database records representing 194 unique articles (D1, pp. 6–9).

Current workbook snapshot
300 included harmonized rows, 203 original record IDs and 193 unique articles. Charts count distinct publication identifiers per category (normalized DOI or SSRN abstract URL). The row marked in the workbook as outside seed priming scope is excluded. Categories are non-exclusive, so totals can exceed the article count.

Molecular evidence

Five D1 domains. Targeted gene expression alone is excluded from the transcriptomics count.

  • Metabolomics88
  • Transcriptomics80
  • Proteomics26
  • Epigenetics14
  • Genomics11

Plant systems

41 of 53 species appear in fewer than five articles. Multi-species cells contribute to each listed species.

  • Oryza sativa24
  • Triticum aestivum24
  • Zea mays17
  • Solanum lycopersicum13
  • Brassica napus10
  • Glycine max10
  • Arabidopsis thaliana9
  • Hordeum vulgare9
  • Sorghum bicolor7
  • Pisum sativum6
More categories
  • Vicia faba · 6
  • Arachis hypogaea · 5
  • Avena sativa · 4
  • Capsicum annuum · 4
  • Gossypium hirsutum · 4
  • Helianthus annuus · 4
  • Triticum durum · 4
  • Cucumis sativus · 3
  • Nicotiana tabacum · 3
  • Phaseolus vulgaris · 3
  • Vigna radiata · 3
  • Camelina sativa · 2
  • Carthamus tinctorius · 2
  • Chenopodium quinoa · 2
  • Daucus carota · 2
  • Lactuca sativa · 2
  • Linum usitatissimum · 2
  • Medicago sativa · 2
  • Ocimum basilicum · 2
  • Artemisia annua · 1
  • Brassica campestris · 1
  • Brassica oleracea · 1
  • Citrullus lanatus · 1
  • Cucurbita pepo · 1
  • Fraxinus excelsior · 1
  • Lens culinaris · 1
  • Medicago truncatula · 1
  • Pancratium maritimum · 1
  • Pennisetum glaucum · 1
  • Physalis angulata · 1
  • Picea abies · 1
  • Poincianella pyramidalis · 1
  • Polygonum persicaria · 1
  • Raphanus sativus · 1
  • Ricinus communis · 1
  • Sesamum indicum · 1
  • Solanum melongena · 1
  • Solanum paniculatum · 1
  • Solanum tuberosum · 1
  • Stevia rebaudiana · 1
  • Trigonella foenum-graecum · 1
  • Valerianella locusta · 1
  • Vigna unguiculata · 1

Priming approaches

Harmonized agent classes. Application methods such as soaking are tracked separately in the source workbook.

  • Nanoparticle44
  • Hormonal35
  • Nutripriming31
  • Signalling molecule priming25
  • Other chemical priming23
  • Antioxidant18
  • Salt17
  • Biopriming7
  • Amino acid6
  • Osmopriming6
More categories
  • Physical · 5
  • Hydropriming · 4
  • Epigenetic priming · 1

Stress conditions

Harmonized stress categories, including control, combined and unreported entries where present.

  • Abiotic – salinity51
  • Abiotic – drought33
  • None / control17
  • Abiotic – heavy metal / toxic ion15
  • Biotic – fungal pathogen14
  • Multiple / combined13
  • Not reported12
  • Abiotic – seed ageing9
  • Biotic – pest / herbivore7
  • Other / unclassified5
More categories
  • Abiotic – cold · 3
  • Abiotic – heat · 3
  • Abiotic – nutrient deficiency · 3
  • Abiotic – submergence / flooding · 3
  • Abiotic – osmotic · 2
  • Abiotic – radiation / light · 2
  • Biotic – bacterial pathogen · 2
  • Abiotic – alkaline · 1
  • Biotic – unspecified · 1

From evidence map to improved experiments

  • Choose the species, developmental stage and stress context before selecting the priming agent.
  • Record concentration, duration, environmental conditions, drying and controls.
  • Specify the experimental unit and match samples and contrasts across omics layers where possible.
  • Connect molecular signals to germination, seedling performance and later agronomic traits.
  • Keep stable publication IDs and track repeated observations separately.

How to use the atlas

Researchers and students
Use the distributions to prioritize candidate evidence gaps for follow-up in the source data.
Educators
Teach the distinction between evidence frequency, mechanism and experimental efficacy.
WG1 and WG2
Choose matched molecular and phenotypic endpoints for joint analyses.
Stakeholders
See where molecular research is concentrated and where further validation is needed.

Source basis · WG1 Deliverable D1, pp. 6–13 and 17–21, as cited in the supplied editorial brief. Figures are recalculated from the Harmonized Data sheet. Species labels use the first two name tokens, with known spelling corrections. Compound priming and omics labels are split. Review new labels during WG1 QA.

Release the searchable record-level database only after QC, licensing/IPR review and approval of source metadata.